| RESEARCH ARTICLE
|Year : 2010 | Volume
| Issue : 2 | Page : 65-69
Immunomodulatory activity of a Chinese herbal drug Yi Shen Juan Bi in adjuvant arthritis
Pathirage Kamal Perera1, Yunman Li1, Cheng Peng1, Weirong Fang1, Caifeng Han2
1 Department of Physiology and Pharmacology, China Pharmaceutical University, Mailbox 207 Tongjiaxiang 24, Nanjing, Jiangsu, 210009, People's Republic of China
2 Jiangsu Chiatai Qingjiang Pharmaceutical Co., Ltd. Huaian Jiangsu, People's Republic of China
Objective: To investigate the immunomodulating mechanisms of a Chinese herbal medicine Yi Shen Juan Bi (YJB) in treatment of adjuvant arthritis (AA) in rats.
Materials and Methods: Levels of serum tumor necrosis factor alpha (TNF-α) and interleukin-1β (IL-1β) were measured by the Enzyme-Linked Immunosorbent Assay (ELISA). Expression of TNF-α mRNA and IL-1β mRNA in synovial cells was measured with the semi-quantitative technique of reverse transcription-polymerase chain reaction (RT-PCR), while caspase-3 was examined by western blot analysis.
Results: The administration of YJB significantly decreased the production of serum TNF-α and IL-1β. It also decreased significantly the TNF-α mRNA, IL-1β mRNA, and caspase-3 expression in synoviocytes.
Conclusions: YJB produces the immunomodulatory effects by downregulating the over-activated cytokines, while it activates caspase-3, which is the key executioner of apoptosis in the immune system. This may be the one of the underlying mechanisms that explains how YJB treats the rheumatoid arthritis.
Department of Physiology and Pharmacology, China Pharmaceutical University, Mailbox 207 Tongjiaxiang 24, Nanjing, Jiangsu, 210009, People's Republic of China
Source of Support: None, Conflict of Interest: None
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