IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 431 
Small font sizeDefault font sizeIncrease font size
Navigate Here
  Search
 
  Next article
  Previous article 
  Table of Contents
  
Resource Links
   Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
   Article in PDF (73 KB)
   Citation Manager
   Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
* Registration required (free)

 
In This Article
   References
   Article Figures

 Article Access Statistics
    Viewed2202    
    Printed71    
    Emailed1    
    PDF Downloaded483    
    Comments [Add]    
    Cited by others 3    

Recommend this journal

 


 
LETTER TO THE EDITOR
Year : 2009  |  Volume : 41  |  Issue : 4  |  Page : 205-206
 

Medicinal plant-based health products: Where is the medicinal constituent?


1 Departments of Pharmacology and Ocular Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, New Delhi, India
2 Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India

Date of Web Publication26-Sep-2009

Correspondence Address:
R Mathur
Departments of Pharmacology and Ocular Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, New Delhi
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.56063

Rights and Permissions



How to cite this article:
Mathur R, Velpandian T. Medicinal plant-based health products: Where is the medicinal constituent?. Indian J Pharmacol 2009;41:205-6

How to cite this URL:
Mathur R, Velpandian T. Medicinal plant-based health products: Where is the medicinal constituent?. Indian J Pharmacol [serial online] 2009 [cited 2020 Aug 9];41:205-6. Available from: http://www.ijp-online.com/text.asp?2009/41/4/205/56063


Sir,

Ancient literatures like Chinese, Unani, Ayurveda, and Siddha extol the virtues of plant-derived medicines. Their preventive and therapeutic benefits have been defined for almost all ailments. The long-standing use of herbal medicines and the belief that they are safe and inexpensive have collectively contributed to their mass appeal. The World Health Organization (WHO) estimates that about 80% of the population in developing countries relies on herbal medicines at least for their primary healthcare and an equal proportion in developed countries have tried this approach at least once. [1] However, there are increasing number of reports in the literature that are questioning the quality of herbal medicines and it is feared that the public is being exposed to a major health risk due to misidentification, adulteration, and contamination of these "health products."

This study was conducted with the aim to bring out the facts by randomly selecting medicinal plant-based products that are available in the market and assess their quality. The products of Ashwagandha (Withania somnifera, Apocynaceae) were chosen, as it is one of the most popular and commonly used medicinal plants. The steroidal lactones (withanolides) present in its roots have been implicated with a wide range of therapeutic activities like anti-cancer, anti-epileptic, memory enhancer, mood elevator, rejuvenator, stress reliever, anti-ageing, anti-oxidant, and in general adaptogenic effect. They have also been identified as "marker compounds" for chemical standardization of Ashwagandha-based products. In this study, a simple high-performance liquid chromatography based method was developed to quantify the withanolide and withaferin in different commercially available Ashwagandha-based products.

The four commercial samples of Ashwagandha were in the form of tablets (samples A and B), powder (sample C), and syrup (sample D). The film coating of the tablets was carefully scraped off and three tablets were triturated. The 1 g of tablet powder was refluxed (methanol, 100 ml), and concentrated to give a methanolic extract. Similarly, the methanolic extract of the powder sample C (1 g) was prepared. Sample D (5 ml) was first lyophilized to give a dry powder, which was extracted using methanol, by the aforementioned method.

Standard withanolide-A (10 mg/ml) and withaferin-A (20 mg/ ml) were prepared and used to set the standard curve. The Nova-pak; column (RP-C18, 4 mm,150 Χ 30 mm i.d.; Waters, Milford, USA) was used as the stationary phase. A freshly filtered mobile phase (potassium-dihydrogen orthophosphate (0.05 M): acetonitrile:3:2, v/v) was used to isocratically elute samples (20 ml) at the flow rate of 1 ml/min for a run time of 25 minutes. The samples were run in triplicate under constant PDA detection, and analytical wavelength was set at 229 nm. All calibration curves of withanolide-A and withaferin-A were made with correlation values of at least 0.995. The inter-day variabilities for withanolide-A and withaferin-A were assayed at concentrations of 0.062-2.0 mg/ml on three sequential days.

Standard withanolide-A and withaferin-A were eluted at a retention time of 3.3 and 5.3 minutes, respectively. In this method, the limit of detection (LOD) was found to be 0.23 and 0.26 mg/ml, respectively.

Although, all the samples tested were polyherbal and had a label claim for the presence of Ashwagandha, only three samples (A, B, and D) had claim for the percentage of Ashwagandha. In addition, only sample B had identified the marker compound (withanolides) and mentioned its adjusted percentage. Further, the labels failed to clarify the amount of withanolides, dosage, indications or storage conditions.

The developed HPLC method gave optimum separation of the constituents of the methanolic extract of the different samples. Under these conditions, base separated peaks of withanolide-A and withaferin-A could be identified and quantified. Chromatogram of the sample B clearly showed base separated peaks of withanolide-A and withaferin-A [Figure 1]. In contrast, the chromatogram of sample D revealed the presence of withanolide-A only [Figure 2]. Using the external calibration method, the concentrations of withanolide-A and withaferin-A were calculated in each sample [Table 1]. The results revealed a wide variation in the quantity of the two chemical constituents present in the products that are available in the market. In sample D, the quantity of withaferin-A could not be assessed as it was below the LOD.

This study highlights the gross liberties enjoyed by the manufacturers of medicinal plant-based products in India. The label claims are inadequate regarding the contents, their quantity, and quality or usage/storage advice of the products. In light of these inconsistencies, the user is being misled regarding the benefit of these products.

Bulk manufacturing of standardized plant-based products is challenging, [2] and in view of this, the United States Congress, passed the Dietary Supplement Health and Education Act. [3],[4] However, this is self-limiting as labeling requirements for dietary supplements are not as stringent as that for pharmaceuticals, and concerns regarding their purity and potency are not reduced. In India and in other developing countries, there is paucity of stringent and effective regulatory control on quality standards for sale of over-the-counter medicinal plant-based products, especially regarding drugs' chemical instability, inappropriate storage, and transport, etc. Under the present scenario, the naοve masses are at the compromising end regarding the safety and efficacy of these "health products." The issue of inconsistency in the chemical constituents of the medicinal plant-based products is a dynamite waiting to explode and needs immediate redress.

 
  References Top

1.Traditional medicine-growing needs and potential. In: WHO policy perspectives on medicines.No. 2: 1-6. Geneva: WHO; 2002.   Back to cited text no. 1      
2.Schuster BG. A new integrated program for natural product development and the value of an ethnomedical approach. J Altern Complement Med 2001;7:S61-72.  Back to cited text no. 2      
3.PL No. 103-417, 108Stat.4325. Dietary Supplement Health and Education Act of 1994.  Back to cited text no. 3      
4.Bass IS, Raubicheck CJ. Marketing dietary supplements. Washington DC: Food and Drug Law Inst. 2000. Available from: http://www.fda. [cited in 2000].  Back to cited text no. 4      


    Figures

  [Figure 1], [Figure 2], [Table 1]

This article has been cited by
1 Prenatal developmental toxicity evaluation ofWithania somniferaroot extract in Wistar rats
P. C. Prabu,S. Panchapakesan
Drug and Chemical Toxicology. 2014; : 1
[Pubmed] | [DOI]
2 Ashwagandha (Withania somnifera): Role in Safeguarding Health, Immunomodulatory Effects, Combating Infections and Therapeutic Applications: A Review
Ruchi Tiwari,Sandip Chakrabort,Mani Saminathan,Kuldeep Dhama,Shoor Vir Singh
Journal of Biological Sciences. 2014; 14(2): 77
[Pubmed] | [DOI]
3 Rapid and sensitive method for determination of withaferin-A in human plasma by HPLC
Pankaj Patial, Vikram Gota
Bioanalysis. 2011; 3(3): 285
[VIEW] | [DOI]



 

Top
Print this article  Email this article

    

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow