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 »  Abstract
 »  Introduction
 »  Materials and Me...
 »  Results
 »  Discussion
 »  References
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RESEARCH ARTICLE
Year : 2009  |  Volume : 41  |  Issue : 3  |  Page : 140-143
 

Mechanisms of potentiation of Angiotensin II-induced contractile response of isolated rat aorta by hydrogen peroxide and tert-butyryl hydroperoxide


1 Department of Pharmacology, Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherva-382711, Gujarat, India
2 Department of Pharmacology and Physiology, UMDNJ - New Jersey Medical School, Medical Sciences Building, 185 S, Orange Avenue, Newark, NJ 07103

Date of Submission21-Feb-2007
Date of Decision18-Feb-2008
Date of Acceptance08-Jun-2009
Date of Web Publication28-Aug-2009

Correspondence Address:
R J Patel
Department of Pharmacology, Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherva-382711, Gujarat, India

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.55208

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 » Abstract 

Objective: To study the mechanism involved in hydrogen peroxide (H 2 O 2 ) or tert-butyl hydroperoxide (t-BHP)-induced potentiation of the Ang II-mediated contraction of isolated rat thoracic aorta.
Materials and Methods: Thoracic aorta was isolated from the Sprauge dawley rats (300-320 gm), cut spirally and response to Ang II (5 ´ 10 -8 M) was taken in the absence and presence of H 2 O 2 (10 -6 M) and t-BHP (10 -5 M). To explore the probable mechanism of H 2 O 2 and t-BHP-induced potentiation of Ang II-mediated contractile response, different blockers such as losartan (AT 1 receptor blocker; 1 µM), catalase (H 2 O 2 scavenger; 500 U/ml), lercanidipine (L-type calcium channel blocker; 1 µM), geinistein (tyrosine kinase inhibitor; 100 µM), and indomethacin (cyclo-oxygenase inhibitor; 10 µM) were used.
Results: In spiral preparation of rat thoracic aorta, H 2 O 2 (10 -6 M) and t-BHP (10 -5 M) did not produce the contraction as such. However, when they are added simultaneously with Ang II (5 ´ 10 -8 M), they potentiated the contractile response of the Ang II. Catalase (500 U/ml) partially antagonized the Ang-II-induced contraction, as well as antagonized the potentiation induced by H 2 O 2 . Losartan (1 µM) and lercanidipine (1 µM) antagonized the Ang II-induced contractile response without affecting H 2 O 2 (10 -6 M)-mediated potentiation. Geinistein (100 µM) antagonized H 2 O 2 (10 -6 M)-mediated potentiation, but it slightly decreased the Ang II response. Losartan (1 µM) and lercanidipine (1 µM) and Geinistein (100 µM) antagonized the Ang II-induced contractile response but not t-BHP-mediated potentiation. Indomethacin antagonized t-BHP-mediated potentiation without affecting much of Ang II response.
Conclusion: From the above-mentioned results, we can reasonably conclude that H 2 O 2 and t-BHP potentiated the contraction induced by the Ang II. H 2 O 2 -induced potentiation of Ang II response may be mediated through tyrosine kinase activation and t-BHP through the activation of cyclo-oxygenase enzyme.


Keywords: Angiotensin II, hydrogen peroxide, reactive oxygen species, tert butyryl hydroperoxide


How to cite this article:
Patel R J, Patel P D, Patel M M, Patel N J, Thyagarajan B. Mechanisms of potentiation of Angiotensin II-induced contractile response of isolated rat aorta by hydrogen peroxide and tert-butyryl hydroperoxide. Indian J Pharmacol 2009;41:140-3

How to cite this URL:
Patel R J, Patel P D, Patel M M, Patel N J, Thyagarajan B. Mechanisms of potentiation of Angiotensin II-induced contractile response of isolated rat aorta by hydrogen peroxide and tert-butyryl hydroperoxide. Indian J Pharmacol [serial online] 2009 [cited 2020 Jun 5];41:140-3. Available from: http://www.ijp-online.com/text.asp?2009/41/3/140/55208



 » Introduction Top


Several intracellular signaling events stimulated by reactive oxygen species (ROS) have been defined, including the activation of mitogen-activated protein kinase family, tyrosine kinases, and different isoenzymes of protein kinase C (PKC) as redox-sensitive kinases. [1],[2],[3] Plethora of literature supported that ROS may directly induce cell proliferation of some cell types. [4],[5],[6]

Ang II directly causes cell proliferation and cell hypertrophy of different cell types, [7] stimulates gene transcription, [8] induces gene expression of enzymes that produce mediators (e.g. NAD(P)H oxidase, phospholipase A and phospholipase D) [9],[10] and also activates multiple intracellular signaling cascades (Tyrosine kinases, Ca 2+ -calmodulin, and mitogen-activated protein (MAP) kinases). Moreover, the hypertrophic effect of the peptide on vascular cells may be prevented by antioxidant treatments. These actions of Ang II have been related to increased ROS synthesis, which is dependent on peptides. [11] Specific studies directed to assess the specific pathway of ROS synthesis activated by Ang II point to H 2 O 2 as the main ROS involved in the hypertrophic response and to the NADH/NADPH oxidase system as the primary target for peptide action.[12] There is only one study, as far as we know, which reported that H 2 O 2 may play a role in Ang II-mediated contraction in vitro ; however, the concentration response relation and underlying mechanism, especially for the potentiation component were not fully documented. [13]

In light of the above observations, the present experiments were designed to study the role of ROS, particularly hydrogen peroxide and t-butyryl hydroperoxide in Angiotensin II-induced contractile response in isolated rat thoracic aorta.


 » Materials and Methods Top


Chemicals

Angiotensin II and catalase were purchased from MP Biomedicals Inc, France; t-BHP and geinistein from Sigma Chemicals Co., St. Louis, USA; H 2 O 2 from Merc, India. All other chemicals were of reagent grade, and were received as gift sample.

Animals

Healthy and adult male Sprague Dawley rats (300-320 g) were procured from Central Animal Facility, SKPCER. They were maintained under controlled temperature and humidity, and standard diet and water was provided ad libitum . The care and the use of these animals were in accordance with the guidelines of the CPCSEA. Institutional Animal Ethics Committee approved the experimental protocols.

Rat thoracic aorta spiral preparation

Rats were sacrificed by cervical dislocation under mild anesthesia and thoracic aorta was isolated from the heart to the diaphragm. It was freed from fat and connective tissues. Care was taken not to stretch the vessel. Helical strips of aorta of 3 mm in width and 20 mm in length were cut with sharp iris scissors and placed in 10-ml organ bath containing a modified Krebs Henseleit solution (KHS; NaCl - 118 mM, KCl - 4.7 mM, KH 2 PO 4 - 1.2 mM, Mg SO 4 .7H 2 O - 1.2 mM, CaCl 2 .2H 2 O - 2.5mM, NaHCO 3 - 25 mM and glucose - 11 mM) of pH 7.4 (at every hour pH was checked and adjusted) and osmolality (280-308 mmol/kg). The solution was continuously aerated with carbogen (95% O 2 + 5% CO 2 ) at 37 °C. A resting tension of 2 g was applied and allowed to equilibrate for 2 h. Changes in the isotonic contraction were recorded on chart recorder using an isotonic fine-movement transducer (Bio Devices, Ambala, India). During equilibration and throughout the experiment, KHS in the organ bath was changed in every fifteen min.

Protocol for vascular reactivity study

After 2 h of equilibration period, two wake up responses of KCl (80 mM) were taken to check the stability of tissue. Responses of Ang II (5 × 10 -8 M) were taken in the absence and presence of H 2 O 2 (10 -6 M) and t-BHP (10 -5 M). For deciphering mechanism behind the potentiation of Ang II by H 2 O 2 and t-BHP, they were added when the Ang II responses had reached the platue in the absence and presence of blocker. The preparation was preincubated with the blocker for 15 min.

Statistical analysis

The responses were expressed as mean th + SEM of % contraction and n represents the number of rats. Statistical analysis was performed by ANOVA. The differences were considered as significant when P <0.05.


 » Results Top


The direct addition of H 2 O 2 (10 -6 M) and t-BHP (10 -5 M) to isolated rat thoracic aorta did not produce contraction excluding their direct contractile effect.

In the present experiments, the contraction by Ang II (5 × 10 -8 M)-induced aorta was used as a control. We studied the contraction of Ang II (5 × 10 -8 M) preincubated aorta with H 2 O 2 (10 -6 M) and t-BHP (10 -5 M). The contraction of aorta elicited by Ang II was augmented after ROS treatment. Catalase (500 U/ml) partially arrested the Ang II-mediated contraction. There was also suppression of the potentiation of Ang II by H 2 O 2 after catalase treatment [Figure 1].

To explore the possible mechanism of H 2 O 2 -induced potentiation of Ang II contractile response, the effect of various blockers to its responses were studied in the absence and the presence of H 2 O 2. Losartan (1 µM) and lercanidipine (1 µM) antagonized the Ang II-induced contractile response, but not antagonized H 2 O 2 -induced potentiation of Ang II contractile response. Geinistein (100 µM) significantly attenuated H 2 O 2 -induced potentiation of Ang II contractile response and also partially antagonized Ang II-induced contractile response [Figure 2].

Similarly, losartan (1 µM) and lercanidipine (1 µM) blocked the Ang II-induced contractile responses, but not t-BHP-induced potentiation of Ang II-induced contractile response. Geinistein (100 µM) failed to suppress t-BHP-induced potentiation of Ang II contractile responses, but it partially antagonized Ang II -induced contractile response. Indomethacin (10 µM) attenuated the t-BHP-induced potentiation without affecting much of Ang II responses [Figure 3].


 » Discussion Top


In the present study, we found that H 2 O 2 and t-BHP potentiated the contractile response of Ang II. This study was designed to explore the probable mechanisms of this potentiation.

The current experimental findings suggest that H 2 O 2 may have a role in the contraction of Ang II in rat thoracic aorta, because catalase completely blocked the H 2 O 2 -induced potentiation of contractile response to Ang II and also suppressed the contractile response induced by Ang II. The best-recognized action of catalase is its ability to remove hydrogen peroxide, [14] and as catalase blocked the Ang II -induced contraction, it can be hypothesized that H 2 O 2 generation is necessary for the Ang II-induced contractile response. The argument, which supports this hypothesis is that the synthesis of H 2 O 2 probably increased after Ang II treatment in aorta may be responsible as exogenously administered H 2 O 2 (10 -6 M), did not induce any contractile response in quiescent aorta, while it potentiated the contractile response of Ang II in precontracted aorta. Previous studies strongly support the idea that the NADH/NADPH oxidase system may be the target for an increased production of hydrogen peroxide after Ang II treatment. [12] The results obtained in the present study may also involve the activation of NADP/NADPH oxidase pathway for Ang II-induced contractile response.

Losartan and lercanidipine produced partial antagonism of the Ang II-induced contractile response, suggesting that the Ang II-induced contraction is dependent not only on the classical pathways described previously but also it may be mediated through intracellular signal transduction, such as the activation of extracellular signal-regulated kinases. [15] Ang II-induced synthesis of hydrogen peroxide could play a central role, because it is well known that this ROS may also activate extracellular signal-regulated kinases. [16]

Losartan and lercanidipine failed to attenuate the H 2 O 2 -induced potentiation of Ang II, suggesting that AT 1 receptor and Ca 2+ channel activation may not be involved in H 2 O 2 -induced potentiation . In quiescent vessel, H 2 O 2 at higher concentration induced vasoconstriction, which may be mediated through activation of phospholipase A 2 , cyclo-oxygenase, phospholipase C, and tyrosine kinase. [17],[18] In the present study, the transient contractile component of Ang II-precontracted thoracic aorta to H 2 O 2, was significantly attenuated by a tyrosine kinase inhibitor, geinistein, suggesting the involvement of tyrosine kinase pathway as mediator in the potentiation activity.

Similarly, losartan and lercanidipine partially antagonized Ang II-induced contractile responses without affecting t-BHP -induced potentiation of Ang II-induced contractile response of rat aorta strip, suggesting that there is no involvement of AT 1 receptor and Ca 2+ channel in t-BHP-induced potentiation. Geinistein also did not modify the t-BHP-induced potentiation of Ang II contractile response. Indomethacin markedly suppressed t-BHP-induced potentiation without antagonism of Ang II-induced contractile response, which supports the work by Garcia-Cohen E.C. et al , 2000. [19] In short, it is demonstrated that H 2 O 2 and t-BHP-induced potentiation of Ang II -induced contractile responses of isolated rat thoracic aorta may involve activation of tyrosine kinase and cyclo-oxygenase pathways, respectively.

 
 » References Top

1.Palmer HJ, Paulson KE. Reactive oxygen species and antioxidants in signal transduction and gene expression. Nutr Rev 1997;55:353-61.  Back to cited text no. 1  [PUBMED]  
2.Finkel T. Signal transduction by reactive oxygen species in non-phagocytic cells. J Leukocyte Biol 1999;65:337-40.  Back to cited text no. 2  [PUBMED]  [FULLTEXT]
3.Sattler M, Winkler T, Verma S, Byrne CH, Shrikhande G, Salgia R, et al. Hematopoietic growth factors signal through the formation of reactive oxygen species. Blood 1999;93:2928-35.  Back to cited text no. 3  [PUBMED]  [FULLTEXT]
4.Duque I, García-Escribano C, Rodríguez-Puyol M, Díez-Marqués ML, López-Novoa JM, Arribas I, et al. Effects of reactive oxygen species on cultured rat mesangial cells and isolated rat glomeruli. Am J Physiol 1992;263:F466-73.  Back to cited text no. 4    
5.Nishio E, Watanabe Y. The involvement of reactive oxygen species and arachidonic acid in alpha 1-adrenoceptor-induced smooth muscle cell proliferation and migration. Br J Pharmacol 1997;121:665-70.  Back to cited text no. 5  [PUBMED]  [FULLTEXT]
6.Sundaresan M, Yu Z, Ferrans VJ, Irani K, Finkel T. Requirement for generation of H2O2 for platelet-derived growth factor signal transduction. Science 1995;270:296-99.  Back to cited text no. 6    
7.Gibbons GH, Pratt RE, Dzau VJ. Vascular smooth muscle cell hypertrophy vs. hyperplasia. Autocrine transforming growth factor-beta 1 expression determines growth response to angiotensin II. J Clin Invest 1992;90:456-61.  Back to cited text no. 7    
8.Taubman MB, Berk BC, Izumo S, Tsuda T, Alexander RW, Nadal-Ginard B. Angiotensin II induces c-fos mRNA in aortic smooth muscle and role of Ca 2+ mobilization and protein kinase C activation. J Biol Chem 1989;264:526-30.  Back to cited text no. 8  [PUBMED]  [FULLTEXT]
9.Schlondorff D, DeCandido S, Satriano JA. Angiotensin II stimulates phospholipases C and A in cultured rat mesangial cells. Am J Physiol 1987;253:113-20.  Back to cited text no. 9    
10.Griendling KK, Minieri CA, Ollerenshaw JD, Alexander RW. Angiotensin II stimulates NADH and NADPH oxidase activation in cultured vascular smooth muscle cells. Circ Res 1994;74:1141-8.  Back to cited text no. 10  [PUBMED]  [FULLTEXT]
11.Rajagopalan S, Kurz S, Munzel P, Tarpey M, Freeman BA, Griendling KK, et al. Angiotensin II -mediated hypertension in the rat increases vascular superoxide production via membrane NADH/NADPH oxidase activation:Contribution to alterations of vasomotor tone. J Clin Invest 1996;97:1916-23.  Back to cited text no. 11    
12.Zafari AM, Fukai MU, Akers M, Yin Q, Shah A, Harrison DG, et al. Role of NADH/NADPH oxidase-derived H2O2 in angiotensin II-induced vascular hypertrophy. Hypertension 1998;32:488-95.  Back to cited text no. 12    
13.Torrecillas G, Boyano-Adánez MC, Medina J, Parra T, Griera M, López-Ongil S, et al. The Role of Hydrogen Peroxide in the Contractile Response to Angiotensin II. Mol Pharmacol 2000;59:104-2.   Back to cited text no. 13    
14.Poduslo JF, Whelan SL, Curran GL, Wengenack TM. Therapeutic benefit of polyamine-modified catalase as a scavenger of hydrogen peroxide and nitric oxide in familial amyotrophic lateral sclerosis transgenics. Ann Neurol 2001;48:943-7.  Back to cited text no. 14    
15.Touyz RM, El Mabrouk M, He G, Wu XH and Schiffrin EL. Mitogen-activated protein/extracellular signal-regulated kinase inhibition attenuates angiotensin II-mediated signaling and contraction in spontaneously hypertensive rat vascular smooth muscle cells. Circ Res 1999;84:505-15.  Back to cited text no. 15    
16.Sabri A, Byron KL, Samarel AM, Bell J, Lucchesi PA. Hydrogen peroxide activates mitogen-activated protein kinases and Na1-H1 exchange in neonatal rat cardiac myocytes. Circ Res 1998;82:1053-62.  Back to cited text no. 16  [PUBMED]  [FULLTEXT]
17.Yang ZW, Zheng T, Zhang A, Altura BT, Altura BM. Mechanism of hydrogen peroxide induced contraction of rat aorta. Eur J Pharmacol 1998;344:169-81.  Back to cited text no. 17  [PUBMED]  
18.Jin N, Rhoades RA. Activation of tyrosine kinase in H 2 O 2 -induced contraction in pulmonary artery. Am J Physiol 1997;212:2686-92.  Back to cited text no. 18    
19.Garcia-Cohen EC, Marin J, Diez-Picazo LD, Baena AB, Salaices M, Rodriguez-Martinez MA. Oxidative stress induced by tert-butyl hydroperoxide causes vasoconstriction in the aorta from hypertensive and aged rats: role of cylooxygenase-2 isoform. J Pharmacol Exp Ther 2000; 293:75-81.  Back to cited text no. 19  [PUBMED]  [FULLTEXT]


    Figures

  [Figure 1], [Figure 2], [Figure 3]

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