| RESEARCH ARTICLE
|Year : 2008 | Volume
| Issue : 4 | Page : 147-151
In vitro antioxidant activity of pet ether extract of black pepper
Ramnik Singh1, Narinder Singh2, BS Saini3, Harwinder Singh Rao1
1 Sri Sai College of Pharmacy, Badhani, Pathankot, Gurdaspur, India
2 Department of Pharmacology, SGRD Medical Institute, Amritsar, India
3 Institute of Pharmaceutical Sciences and Engineering, Amritsar, India
Objective: To investigate the in vitro antioxidant activity of different fractions (R1, R2 and R3) obtained from pet ether extract of black pepper fruits (Piper nigrum Linn.)
Materials and Methods: The fractions R1, R2 and R3 were eluted from pet ether and ethyl acetate in the ratio of 6:4, 5:5 and 4:6, respectively.
1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, superoxide anion radical, nitric oxide radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the extract.
Results: The free radical scavenging activity of the different fractions of pet ether extract of P. nigrum (PEPN) increased in a concentration dependent manner. The R3 and R2 fraction of PEPN in 500 µg/ml inhibited the peroxidation of a linoleic acid emulsion by 60.48±3.33% and 58.89±2.51%, respectively. In DPPH free radical scavenging assay, the activity of R3 and R2 were found to be almost similar. The R3 (100µg/ml) fraction of PEPN inhibited 55.68±4.48% nitric oxide radicals generated from sodium nitroprusside, whereas curcumin in the same concentration inhibited 84.27±4.12%. Moreover, PEPN scavenged the superoxide radical generated by the Xanthine/Xanthine oxidase system. The fraction R2 and R3 in the doses of 1000µg/ml inhibited 61.04±5.11% and 63.56±4.17%, respectively. The hydroxyl radical was generated by Fenton's reaction. The amounts of total phenolic compounds were determined and 56.98 µg pyrocatechol phenol equivalents were detected in one mg of R3.
Conclusions: P. nigrum could be considered as a potential source of natural antioxidant.
Sri Sai College of Pharmacy, Badhani, Pathankot, Gurdaspur
Source of Support: None, Conflict of Interest: None
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