|Year : 2008 | Volume
| Issue : 3 | Page : 107-110
Immunomodulatory effect of Tinospora cordifolia extract in human immuno-deficiency virus positive patients
MV Kalikar1, VR Thawani1, UK Varadpande2, SD Sontakke1, RP Singh3, RK Khiyani4
1 Department of Pharmacology, Government Medical College, Nagpur - 440 003, India
2 Department of Preventive and Social Medicine, Government Medical College, Nagpur - 440 003, India
3 Department of Skin and Venereal Diseases, Government Medical College, Nagpur - 440 003, India
4 Department of Rasa Shastra, Government Ayurvedic College, Nagpur- 440009, India
|Date of Submission||17-Nov-2006|
|Date of Decision||27-Apr-2008|
|Date of Acceptance||21-Jun-2008|
V R Thawani
Department of Pharmacology, Government Medical College, Nagpur - 440 003
Objectives: To assess the safety and efficacy of TCE in human immuno-deficiency virus positive patients.
Materials and Methods: Efficacy of Tinospora cordifolia extract (TCE) in HIV positive patients was assessed in randomized double blind placebo controlled trial. 68 HIV positive participants were randomly assigned to two groups to receive either TCE or placebo for six months. After clinical examination TLC, DLC, ESR, platelet count, hemoglobin and CD4 count were done. The hematological investigations were repeated at bimonthly intervals and CD4 count was repeated at the end of the study. Patients were clinically reviewed at monthly intervals for compliance, refill and ADR monitoring. The drugs were decoded at the end of the trial.
Results: TCE treatment caused significant reduction in eosinophil count and hemoglobin percentage. 60% patients receiving TCE and 20% on placebo reported decrease in the incidence of various symptoms associated with disease. Some of the common complaints reported by patients on TCE were anorexia, nausea, vomiting and weakness.
Conclusion: Tinospora cordifolia extract, a plant derived immunostimulant, significantly affected the symptoms of HIV. This was validated by clinical evaluation. However not all of the objective parameters studied by us, back this up. Tinospora cordifolia could be used as an adjunct to HIV/AIDS management.
Keywords: CD4 count, herbal extract
|How to cite this article:|
Kalikar M V, Thawani V R, Varadpande U K, Sontakke S D, Singh R P, Khiyani R K. Immunomodulatory effect of Tinospora cordifolia extract in human immuno-deficiency virus positive patients. Indian J Pharmacol 2008;40:107-10
|How to cite this URL:|
Kalikar M V, Thawani V R, Varadpande U K, Sontakke S D, Singh R P, Khiyani R K. Immunomodulatory effect of Tinospora cordifolia extract in human immuno-deficiency virus positive patients. Indian J Pharmacol [serial online] 2008 [cited 2013 Dec 7];40:107-10. Available from: http://www.ijp-online.com/text.asp?2008/40/3/107/42302
| » Introduction|| |
Acquired immuno-deficiency syndrome (AIDS) is a fatal illness caused by human immuno-deficiency virus (HIV), which breaks down the host immune system, leaving the subject vulnerable to life-threatening opportunistic infections, neurological disorders and malignancies. Once infected, it is probable that a person will be infected for life. In 2001, 40 million people were living with HIV/AIDS worldwide, prevalence rate was 1.2%, about 5 million became infected and about 3 million died of it.  With high fatality, significant impact on quality of life, lack of curative treatment or vaccine, HIV/AIDS has become a serious global health problem.
The main cause of immune defect in AIDS is deficiency of the thymus-derived lymphocytes (T 4 ), characterized by the presence of CD 4 surface molecules, which are the cellular receptors for HIV.  Anti-retroviral therapy (ART) has issues associated with availability, affordability, toxicity, ADR monitoring and emerging resistance. In the absence of effective cure, immunorestorative therapy seems logical adjunct. It has been suggested that when routinely used pharmacological interventions fail to relieve the symptoms, immunotherapy may be effective.  It is therefore worth looking for a natural product that is safer, affordable, effective, better tolerated and devoid of side effects.
Tinospora cordifolia (Wild) Miers is a deciduous climbing shrub indigenous to tropical Indian subcontinent, belonging to the family Menispermaceae. In Ayurveda Tinospora cordifolia (TC) is used as " rasayana" which has powerful immunostimulant activity.  Charaka described rasayana as antiaging, which increased the life span, promoted intelligence, improved memory and ensured freedom from diseases, indicating immunostimulant effect. 
Pretreatment with TC afforded protection against induced infections in mice  and rats.  In patients with obstructive jaundice, addition of TC to routine surgical procedure increased the survival rate and also increased polymorphonuclear leukocyte functions suggesting an increase in phagocytosis and intracellular killing capacity.  TC extract (TCE) in the same dose as used in this study, showed significant beneficial effect in allergic rhinitis , and burns.  Hence this study was planned to scientifically assess its efficacy in HIV positive patients.
| » Materials and Methods|| |
This was a prospective, randomized, double blind, placebo controlled comparative trial carried out in the AIDS counseling center of Skin and Venereal diseases department of Government Medical College Hospital, Nagpur with permission from Institutional Ethics Committee. Patients diagnosed to be HIV positive were approached with the request to participate in this trial. Those who showed interest were given detailed information handouts about the trial, in the language understood by them. From those who volunteered to participate, informed witnessed written consent was taken. The inclusion and exclusion criteria were:
Subjects diagnosed to be HIV positive
Attending the HIV counseling center of GMCHN
Volunteering to participate and give informed written consent
Of either gender in the age group of 18 - 50 years.
Patients on ART
Suffering from associated diseases like hypertension, ischemic heart disease, diabetes, or CNS disorders.
Clinical evidence of end organ damage
Pregnant or lactating women
Participation in any medicine trial in previous one month.
Baseline pulse, respiratory rate, blood pressure and temperature were recorded. After clinical examination, 68 participants were entered in the trial. They were randomly assigned to two groups of 34 each to receive either coded TCE 300 mg per tablet or matching placebo in the same formulation, packing, size, weight, color and dose of one tablet three times a day for six months. Baseline TLC, DLC, ESR, platelet count, hemoglobin and CD 4 count were done.
The medicines were issued to the participants for one month at a time and refill was given during monthly recall. During recall the participants were asked to bring the leftover medicines along with the container. 80% drug consumption was considered to be compliant. All the returned medicines were discarded. At each monthly visit the patients were asked to report the occurrence of fever, cough, easy fatigability, arthralgia, oral ulcers, diarrhea, skin rash or any other symptom. The participants were at liberty to withdraw from the trial at any time. The participants were explained to take treatment whenever necessary, for minor complaints like fever, diarrhea, skin diseases, pain and also for other infections like tuberculosis.
The TLC, DLC, ESR, platelet count, Hb% were repeated at bimonthly intervals. CD 4 count was repeated at the end of the study. The trial medicines were decoded at the end of the trial and the participants were followed up for additional one month for monitoring the residual effects, if any. Institutional ADR monitoring and reporting system was followed. All data was logged in the case record form by principal investigator. The results were analyzed by Kruskal Wallis test.
M/s Pharmanza India Ltd, Gujarat, India supplied the TCE (Tinofend R ) and matching placebo, free of cost, on our request. Each tablet of Tinofend contained 300 mg of standardized extract obtained from water extract of stem of TC. It contained more than 5% bitter principles and was tested for presence of cordioside and tinosporoside by HPLC.
| » Results|| |
In study participants, HIV was more common in the 18-30 years age group. Half of the enrolled patients were from this age group, 42% from 30-40 years, and 8% from 40-50 years age group. The men: women ratio was 1: 1.06. All had contacted the HIV infection through sexual contact. None of the participants was commercial sex worker. Among the participants were 9 couples. The family history of the participants revealed that spouses of 15 participants (not included in this study) were infected with HIV/AIDS.
Kruskal Wallis H test was used for determining statistical significance. There was no significant difference in the baseline parameters between the two treatment groups [Table 1].
In participants who received TCE, a significant reduction in Hb% and eosinophil count was seen after 6 months of treatment. Statistically significant difference was not seen in other studied parameters [Table 2].
Among the participants who received placebo, there was no difference in CD4 count before and after six months. There was statistically significant reduction in TLC, neutrophil and eosinophil count. The mean lymphocyte count at the end of treatment was significantly higher as compared to baseline [Table 3]. No statistically significant difference was noted between the two groups [Table 4].
During the monthly recalls, participants were clinically examined and evaluated. From the TCE treated group, 60% reported decrease in symptoms, 30% reported no change and 10% patients reported deterioration. On the other hand in the placebo group, 40% patients reported an increase in symptoms, 40% patients reported no change and 20% patients reported decrease in symptoms.
Out of 68 participants entered in the trial, 8 dropped out. On decoding, 5 of them were found to be on placebo and 3 on TCE. Those on placebo did not wish to continue because of lack of relief from symptoms. Those on TCE dropped out due to complaints of anorexia, nausea, vomiting and weakness. One patient receiving TCE died during the trial.
| » Discussion|| |
Pharmaceutical companies like Merind, Himalaya, Krauter market TC in India, either singly or in combination with other herbs, and all recommend these formulations for immunostimulant action. However we could not find any report relating to TC in HIV/ AIDS patients. Hence we lack the data of other studies for comparison.
Other studies have reported that mice treated with TC showed significantly greater phagocytic and intercellular bactericidal capacity of polymorphs.  TCE stimulated peritoneal macrophage in a dose-dependent manner.  TC caused increase in phagocytosis and intracellular killing capacity as suggested by the increase in survival rate and polymorphonuclear leucocyte function when TC was added to routine surgical procedure in patients with obstructive jaundice.  In patients of allergic rhinitis, TCE improved the symptoms of nasal discharge, sneezing, nasal pruritus and nasal obstruction and increase the total leucocyte count.  With such illustrious efficacy reports we expected TCE to show some action in HIV/AIDS. As one of the prime parameters for this we used the CD4 count on which TCE did not show any significant effect.
In our study there was significant reduction in the leukocyte count in participants receiving placebo, while in those receiving TCE there was no significant reduction. The decrease in TLC in placebo group may be due to the natural course of the disease, while in TCE group, the fall in leukocyte count, may have been checked by virtue of its "rasayana" effect. We also noted significant reduction in the eosinophil count with six-month treatment with TCE. Reduction in eosinophils has been reported with immunotherapy.  This supports the immunomodulatory action of TCE.
It has been reported that TCE is also capable of stimulating B-lymphocytes, macrophages and polymorphonuclear leucocytes.  But in our study the mean lymphocyte count in patients receiving TCE did not change significantly, while in the placebo group it showed significant increase, for which we do not have explanation.
Even though we did not get appreciable positive results with TCE on the hematological parameters, the patients on TCE reported significant symptomatic improvement, which correlated with clinical evaluation. This was evident from the requests from most of the participants of TCE group to continue providing them TCE even after conclusion of the trial. However we do realize the limitation of scientific validity of subjective benefits.
Three out of the 34 patients on TCE complained of ADRs like anorexia, nausea, vomiting. In the rest TCE was well tolerated. The patient who died during trial was on TCE. His CD4 count was 245 at baseline. There is no conclusive evidence that his death was as a result of intervention or natural course of the disease. It is known that HIV infection progresses at a very slow pace and may take nearly a decade for full-blown AIDS to develop.
Not all research results in positive findings and this one unfortunately is among those. We feel that six months may not have been enough for studying the efficacy of TCE on HIV positive patients.
| » Acknowledgment|| |
We are grateful to Sir Gangadharrao Chitnavis Memorial Medical Research Trust of Nagpur for funding this study under late Madhavrao Chitnavis Clinical Research Fellowship to principal investigator (MVK). We express our gratitude to Dr. Lal Hingorani of Pharmanza (India), for providing the trial medicines. We are thankful to Dr. M. V. Murhekar, Deputy Director, National Institute of Epidemiology, Chennai, for help in statistical analysis. From Government Medical College Nagpur we thank Mrs. K. J. Gharpure, Research Pharmacist for internally peer reviewing the manuscript, Dr. Sushil Makhija, Lecturer in Microbiology for assistance in lab work, Dr. Kavita Jaiswal, Lecturer in Pharmacology for valuable help in carrying this study.
| » References|| |
|1.||Park's textbook of preventive and social medicine. 18 th ed. Jabalpur, India: M/s Banarasidas Bhanot Publishers; 2005. p. 271. |
|2.||Anthony S, Fauci H, Clifford L. Acquired Immunodeficiency Syndrome (AIDS). In Harrison's principle of internal medicine. Vol. 1, 16 th ed. McGraw Hill: Medical Publishing Division; 2005. p. 1086. |
|3.||Kalra S, Wadhwa P, Kalra N. Rhinitis: Recent advances. Hosp Today 2002;7:461-3. |
|4.||Thatte UM, Dahanukar SA. The Rasayana concept: Clues from immunomodulatory therapy. In: Upadhayaya SN, editor. Immunomodulation. New Delhi, India: Narosa Publishing House; 1997. p. 141-8. |
|5.||Sharma PV. Charak S. Chikitsasthana. Vol 2. Varanasi, India: Chaukhamba Orientalia; 1981. p. 62-74. |
|6.||Thatte UM, Kulkarni, MR, Dahanurkar SA. Immunotherapeutic modification of E. coli peritonitis and bacteremia by Tinospora cordifolia. J Postgrad Med 1992;38:13-5. |
|7.||Dahanukar SA, Thatte UM, Pai NR, More PB, Karandikar SM. Immunotherapeutic modification by Tinospora cordifolia of abdominal sepsis induced by caecal ligation in rats. Indian J Gastroenterol 1988;7:21-3. |
|8.||Bapat RD, Rege NN, Koti R, Desai NK, Dahanukar SA. Can we do away with percutaneous trans hepatic biliary drainage? HPB Surg 1995;9:5-11. |
|9.||Badar VA, Thawani VR, Wakode PT, Shrivastava MP, Gharpure KJ, Hingorani LL, et al. Efficacy of Tinospora cordifolia in allergic rhinitis. J Ethnopharmacol 2004;96:445-9. |
|10.||Badar VA, Thawani VR, Wakode PT, Shrivastava MP, Gharpure KJ, Hingorani LL, et al. Jury still out on the usefulness of a traditional herbal medicine for allergic rhinitis. Focus Alternative Compl Ther 2005;10:188-9. |
|11.||Gangrade G, Thawani V, Sagdeo V, Ughade S, Khiyani R. Efficacy of Tinospora cordifolia on thermal burn patients. Indian Med Gazette 2006;140:38-43. |
|12.||Rege NN, Dahanukar SA. Quantitation of microbicidal activity of mononuclear phagocytes: An in-vitro technique. J Postgrad Med 1993;39:22-5. |
|13.||Furin MJ, Norman PS, Creticos PS. Immunotherapy decreases antigen induced eosinophil cell migration into the nasal cavity. J Allergy Clinical Immunol 1991;88:27-32. |
|14.||Sainis KB, Sumariwalla PF, Goel A, Chintawar GL, Sipahimalani AT, Banerji A. Immunomodulation. New Delhi, India: Narosa Publishing House; 1997. p. 156-87. |
[Table 1], [Table 2], [Table 3], [Table 4]
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