IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 1984 
Small font sizeDefault font sizeIncrease font size
Navigate Here
  Search
 
 » Next article
 » Previous article 
 » Table of Contents
  
Resource Links
 »  Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »  Article in PDF (129 KB)
 »  Citation Manager
 »  Access Statistics
 »  Reader Comments
 »  Email Alert *
 »  Add to My List *
* Registration required (free)

 
In This Article
 »  Abstract
 »  References
 »  Article Tables

 Article Access Statistics
    Viewed6614    
    Printed241    
    Emailed4    
    PDF Downloaded354    
    Comments [Add]    
    Cited by others 16    

Recommend this journal

 


 
RESEARCH PAPER
Year : 2007  |  Volume : 39  |  Issue : 1  |  Page : 30-32
 

Evaluation of the female reproductive toxicity of aqueous extract of Labisia pumila var. alata in rats


1 Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia
2 School of Health Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia

Date of Submission11-Aug-2005
Date of Decision22-Sep-2005
Date of Acceptance29-Jan-2006

Correspondence Address:
S Siti Amrah
Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian, Kelantan
Malaysia
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.30760

Rights and Permissions

 » Abstract 

Objective: To detect potential adverse effects of aqueous extract of Labisia pumila var. alata (LPE) or `Kacip Fatimah' on the estrous cycle, reproductive performance, post-natal growth and offspring survival of rats. Materials and Methods: Forty eight (48) female Sprague Dawley rats with consecutive 4 to 6 days estrous cycle were given distilled water (as control) or LPE at 2, 20, 200, 400 or 800 mg/kg daily by gavaging ten days prior to mating, mating (a maximum period of ten days), gestation and lactation periods of seven days. Dams and fetuses were sacrificed on day seven postnatal. Results: Labisia pumila extracts did not alter the estrous cycle and general health of all rats. All the animals proceed towards successful mating and pregnancies. There was no significant difference in the duration of pregnancy and all pregnant rats delivered normally. Statistically no test agent-related changes in the maternal body weight, number of implantations, litter size and pup body weights were observed. Other parameters measured include pup sex ratio, live birth index, pup viability index and percentage of implantation death which also showed no significant difference. Conclusion: The present findings indicate that water based extracts of Labisia pumila var. alata do not pose any significant reproductive toxicity or complication in pregnancy and delivery in rats. The extract did not significantly alter the duration of pregnancy in rats, however the duration of delivery was not evaluated in this study.


Keywords: Herb, adverse effect, estrous cycle, pregnancy, lactation


How to cite this article:
Wan Ezumi M F, Amrah S S, Suhaimi A, Mohsin S. Evaluation of the female reproductive toxicity of aqueous extract of Labisia pumila var. alata in rats. Indian J Pharmacol 2007;39:30-2

How to cite this URL:
Wan Ezumi M F, Amrah S S, Suhaimi A, Mohsin S. Evaluation of the female reproductive toxicity of aqueous extract of Labisia pumila var. alata in rats. Indian J Pharmacol [serial online] 2007 [cited 2019 Nov 19];39:30-2. Available from: http://www.ijp-online.com/text.asp?2007/39/1/30/30760


Labisia pumila var. alata (LPA ) or popularly known in Malaysia as Kacip Fatimah (KF), is a very popular herb amongst the local womenfolk. Traditionally, the water decoction of the root or whole plant of KF are consumed by the Malay women for induction and facilitation of labour.[1] Currently there are many commercial products containing this herb have emerged in Malaysian market for the purpose of enhancing vitality and libido, however, there is no scientific data on their quality, safety, and efficacy to substantiate claims. Studies supported by the Government of Malaysia, involving this herb conducted at various universities and institutes are in various stages, like extract preparation-standardization (undergoing patenting), authentication, and evaluation of safety and efficacy. Reports have shown that the LPA displayed a non-significant response to in vitro estrogen activity[2] and had appreciable amount of iron.[3] In addition, LPA root and leaves were found to contain two novel benzoquinoid compounds 1, 2 as major components.[3] More information regarding this herb is expected to be available in the near future.

The objectives of the present study are to evaluate the female reproductive toxicity and potential effect of KF in inducing labor in rats. Standardized aqueous extract of Labisia pumila var. alata (LPE) at doses of 2-800 mg/kg/day were administered to determine the safety and efficacy of this herb. The general acute and sub-acute (28 days) toxicity studies of the same extract in rats were already performed by a team in Herbal Medicine Research Center of Institute for Medical Research, Kuala Lumpur, Malaysia. The results of the study revealed that the estimated LD 50 of the extract is more than 5 g/kg BW and the extract produced no significant adverse effects (personal communication). A chronic toxicity study is on going and at present, no deleterious effects is observed in rats. A phase 2 clinical trial conducted in post-menopausal women by a research team at School of Medical Sciences, Universiti Sains, Malaysia concluded that the therapeutic dose of the extract is 2.5 mg/kg/day (personal communication).

About 48 female Sprague Dawley rats were used in this Segment I (female reproductive toxicity) study. Rats with regular 4-6 days estrous cycle were given vehicle (distilled water) as control or LPE at 2, 20, 200, 400, or 800 mg/kg daily, by gavage, 10 days prior to mating, during mating (a maximum period of 10 days), throughout gestation and lactation periods of 7 days. Dams were permitted to deliver their litters naturally. At birth (day 1), pups were individually counted, weighed, and examined for external malformation and sexed. Dams and fetuses were sacrificed on day seven postpartum.[4] The parameters measured are presented in [Table - 1]. Data were analyzed using SPSS version 11.0. Data on maternal body weight throughout the study was analyzed by General Linear Model Repeated Measures. Mean days of estrous cycle, length of pregnancy (days), pregnancy index, number of pups both at birth and on D7 lactation (litter size), pups body weight during lactation and number of implantation sites per litter were analyzed by one-way anova, followed by Scheffe test if differences were found. Additionally, Kruskal Wallis test (non-parametric) followed by Mann-Whitney test (when appropriate) were used to assess the live birth index, viability index and percentage of post-implantation death. The level of significance was set at 5%. The parametric data were expressed as Mean ± Standard error of mean (SEM) or ratio while the non-parametric data were expressed as median (Inter quartile Range) (IQR).

Results indicated that LPA extracts did not alter the general health or estrous cycle of rats. All studied animals proceed toward successful mating and pregnancies. The mean duration of pregnancy (in days) was shortened to 21 days in animals that received the herbal extract at a dose of 20 mg/kg/day and above however, it was not statistically significant. All pregnant rats were delivered normally with no evidence of prematurity or abortion suggesting that the extract is not an abortifacient or having prostaglandin like activity when consumed orally. None of the rats exhibited significant amount of fetal resorption indicating that the herb was nontoxic to the fetuses. Statistically no test agent-related changes in the maternal body weight, number of implantations, litter size, and pup body weights were observed. No significant difference in pup sex ratio, live birth index, pup viability index and percentage of post implantation death were noted in this study.

The present findings indicated that water based extracts of Labisia pumila var. alata do not pose any significant reproductive toxicity or complication in pregnancy, delivery and early pup growth in rats. The no observable adverse effect level (NOAEL) of the extract in this study is 800 mg/kg/day. A closer observation on the duration of pregnancy (in hours) and parturition time were not evaluated in this study to support the traditional claim of this herb.

 
 » References Top

1.Jaganath IB, Ng LT. Herbs: The green pharmacy of Malaysia. Kuala Lumpur: Vinpress Sdn. Bhd in collaboration with the Malaysian Agricultural Research and Development (MARDI): 2000. p. 53-4.  Back to cited text no. 1    
2.Jamal JA, Houghton PJ. Testing of Labisia pumila for oestrogenic activity using a recombinant yeast screen. J Pharma Pharmacol 1998;50:79.  Back to cited text no. 2    
3.Houghton PJ, Jamal JA, Milligan S. Studies on Labisia pumila herb and its commercial products. J Pharma Pharmacol 1999;51:236.  Back to cited text no. 3    
4.Manson JM, Kang YJ. Test methods for assessing female reproductive and developmental toxicology. In : Hayes AW, editor. Principles and methods of toxicology. 3rd ed. Raven Press Ltd: New York; 1994. p. 1026-33.  Back to cited text no. 4    


    Tables

[Table - 1], [Table - 2]

This article has been cited by
1 Time and dose-dependent effects of Labisia pumila on the bone strength of postmenopausal osteoporosis rat model
Nadia Mohd Effendy,Shahrum Abdullah,Mohd Faridz Mod Yunoh,Ahmad Nazrun Shuid
BMC Complementary and Alternative Medicine. 2015; 15(1)
[Pubmed] | [DOI]
2 Allocation of Secondary Metabolites, Photosynthetic Capacity, and Antioxidant Activity of Kacip Fatimah (Labisia pumila Benth) in Response to and Light Intensity
Mohd Hafiz Ibrahim,Hawa Z. E. Jaafar,Ehsan Karimi,Ali Ghasemzadeh
The Scientific World Journal. 2014; 2014: 1
[Pubmed] | [DOI]
3 Labisia pumila regulates bone-related genes expressions in postmenopausal osteoporosis model
Siti Fathilah,Norazlina Mohamed,Norliza Muhammad,Isa Mohamed,Ima Soelaiman,Ahmad Shuid
BMC Complementary and Alternative Medicine. 2013; 13(1): 217
[Pubmed] | [DOI]
4 Labisia pumila protects the bone of estrogen-deficient rat model: A histomorphometric study
Siti Noor Fathilah,Ahmad Nazrun Shuid,Norazlina Mohamed,Norliza Muhammad,Ima Nirwana Soelaiman
Journal of Ethnopharmacology. 2012; 142(1): 294
[Pubmed] | [DOI]
5 Review on Labisia pumila (Kacip Fatimah): Bioactive phytochemicals and skin collagen synthesis promoting herb
Lee Suan Chua,Sze Yean Lee,Norhanisah Abdullah,Mohamad Roji Sarmidi
Fitoterapia. 2012; 83(8): 1322
[Pubmed] | [DOI]
6 The Anti-Inflammatory, Phytoestrogenic, and Antioxidative Role of Labisia pumila in Prevention of Postmenopausal Osteoporosis
M. E. Nadia,A. S. Nazrun,M. Norazlina,N. M. Isa,M. Norliza,S. Ima Nirwana
Advances in Pharmacological Sciences. 2012; 2012: 1
[Pubmed] | [DOI]
7 Labisia pumilaPrevents Complications of Osteoporosis by Increasing Bone Strength in a Rat Model of Postmenopausal Osteoporosis
Siti Noor Fathilah,Shahrum Abdullah,Norazlina Mohamed,Ahmad Nazrun Shuid
Evidence-Based Complementary and Alternative Medicine. 2012; 2012: 1
[Pubmed] | [DOI]
8 The Effect ofLabisia pumilavar.alataon Postmenopausal Women: A Pilot Study
Azidah Abdul Kadir,Nik Hazlina Nik Hussain,Wan Mohammad Wan Bebakar,Dayang Marshitah Mohd,Wan Mohd Zahiruddin Wan Mohammad,Intan Idiana Hassan,Norlela Shukor,Nor Azmi Kamaruddin,Wan Nazaimoon Wan Mohamud
Evidence-Based Complementary and Alternative Medicine. 2012; 2012: 1
[Pubmed] | [DOI]
9 Alkyl phenols and saponins from the roots of Labisia pumila (Kacip Fatimah)
Zulfiqar Ali, Ikhlas A. Khan
Phytochemistry. 2011;
[VIEW] | [DOI]
10 The effects of Labisia pumila var. alata on bone markers and bone calcium in a rat model of post-menopausal osteoporosis
Shuid, A.N., Ping, L.L., Muhammad, N., Mohamed, N., Soelaiman, I.N.
Journal of Ethnopharmacology. 2011; 133(2): 538-542
[Pubmed]
11 The effects of Labisia pumila var. alata on bone markers and bone calcium in a rat model of post-menopausal osteoporosis
Ahmad Nazrun Shuid,Leong Lee Ping,Norliza Muhammad,Norazlina Mohamed,Ima Nirwana Soelaiman
Journal of Ethnopharmacology. 2011; 133(2): 538
[Pubmed] | [DOI]
12 Labisia pumila extract protects skin cells from photoaging caused by UVB irradiation
Hyun-kyung Choi,Dong-hyun Kim,Jin Wook Kim,Sulaiman Ngadiran,Mohamad Roji Sarmidi,Chang Seo Park
Journal of Bioscience and Bioengineering. 2010; 109(3): 291
[Pubmed] | [DOI]
13 Labisia pumila extract protects skin cells from photoaging caused by UVB irradiation
Choi, H.-k., Kim, D.-h., Kim, J.W., Ngadiran, S., Sarmidi, M.R., Park, C.S.
Journal of Bioscience and Bioengineering. 2010; 109(3): 291-296
[Pubmed]
14 Antioxidative properties of leaf extracts of a popular Malaysian herb, Labisia pumila
Norhaiza, M., Maziah, M., Hakiman, M.
Journal of Medicinal Plant Research. 2009; 3(4): 217-223
[Pubmed]
15 Sub-acute toxicity evaluation of an aqueous extract of Labisia pumila, a Malaysian herb
G.D. Singh,M. Ganjoo,M.S. Youssouf,A. Koul,R. Sharma,S. Singh,P.L. Sangwan,S. Koul,D.B. Ahamad,R.K. Johri
Food and Chemical Toxicology. 2009; 47(10): 2661
[Pubmed] | [DOI]
16 Sub-acute toxicity evaluation of an aqueous extract of Labisia pumila, a Malaysian herb
Singh, G.D., Ganjoo, M., Youssouf, M.S., Koul, A., Sharma, R., Singh, S., Sangwan, P.L., Johri, R.K.
Food and Chemical Toxicology. 2009; 47(10): 2661-2665
[Pubmed]



 

Top
Print this article  Email this article

    

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow