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RESEARCH LETTER
Year : 2005  |  Volume : 37  |  Issue : 6  |  Page : 406-407
 

Antiulcer activity of digitrall: A polyherbal drug in rats


1 Department of Pharmacology, Dr. B.C. Roy Institute of Post Graduate Medical Education & Research, Kolkata. J.B. Roy State Ayurvedic Medical College & Hospital, Kolkata., India
2 Department of Pharmacology, Dr. B.C. Roy Institute of Post Graduate Medical Education & Research, Kolkata , India
3 J.B. Roy State Ayurvedic Medical College & Hospital, Kolkata , India

Correspondence Address:
T K Sur
Department of Pharmacology, Dr. B.C. Roy Institute of Post Graduate Medical Education & Research, Kolkata
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.19084

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How to cite this article:
Jana U, Bhattacharyya D, Bandopadhyay S, Sur T K, Pandit S, Debnath P K. Antiulcer activity of digitrall: A polyherbal drug in rats. Indian J Pharmacol 2005;37:406-7

How to cite this URL:
Jana U, Bhattacharyya D, Bandopadhyay S, Sur T K, Pandit S, Debnath P K. Antiulcer activity of digitrall: A polyherbal drug in rats. Indian J Pharmacol [serial online] 2005 [cited 2019 Nov 16];37:406-7. Available from: http://www.ijp-online.com/text.asp?2005/37/6/406/19084


In the last few years, efforts have been taken to identify new antiulcer drugs from natural sources. Plants are the sources of certain known antiulcer drugs. Digitrall (DG) is a polyherbal formulation (prepared by M/s. S.C. Pharmaceuticals Ltd., Kolkata, India) which contains aqueous extracts of Zingiber officinale, Amomum sabulatum, Berberis aristata, Piper nigram, Ptychotis ajowan, Carica Papay and Foenieulum vulgar . Zingiber officinale have antiinflammatory, antiemetic and carminative action, [1],[2] Berberis aristata and Amomum sabulatum have beneficial role in gastroduodenal ulcer and hepatitis,[3] Ptychotis ajowan and Amomum sabulatum have antiemetic activities,[4] Piper nigram and Carica Papay have roles in various gastric ailments,[5] and Foenieulum vulgare has beneficial role in anorexia.[4] Thus, DG is claimed to be useful in gastroduodenal ulcers and indigestion. However, the pharmacological effects need experimental evidence for their actions. The aim of the present study was to evaluate the effect of DG on the prevention of gastric ulcers in rats.

The DG was prepared and supplied by M/s. S.C. Pharmaceuticals Private Limited, Kolkata, in a liquid form. Chemicals and reagents such as indomethacin, superoxide dismutase (SOD), reduced glutathione (GSH), bovine serum albumin, 5-5'-dithio-bis 2-nitrobenzoic acid (DTNB), epinephrine hydrochloride and thiobarbituric acid (TBA), etc. were purchased from Sigma Chemical Co., USA.

A total of 36 male Wistar rats (150-225 g, b.w.) were divided into six groups (n = 6): Group I (5 ml/kg saline), Group II (5 ml/kg, saline), Group III (1 ml/kg digitrall), Group IV (2 ml/kg digitrall), Group V (4 ml/kg digitrall) and Group VI (50 mg/kg ranitidine). All the drugs were administered orally, daily for 10 days, before gastric ulcers were induced in these rats (except Group I), with indomethacin (20 mg/kg) orally. All animals were killed under ether anesthesia after 3 h. of administration of indomethacin. The stomach of each animal was incised along the greater curvature for the examination of ulcers. The glandular part of the stomach was scrapped, homogenized in cold 0.9% saline and centrifuged at 3000 r.p.m. for 15 min. Malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH) and protein were estimated from the supernatant.

The results of all the assays are reported as mean±SEM. Statistical significance was determined using one-way ANOVA followed by Duncan's test or Neuman-Keul test. P<0.05 was considered significant.

By directly inhibiting cyclooxygenase enzymes, indomethacin is indirectly responsible for the overproduction of leukotrienes and 5-lipoxygenase, which are the prime agents responsible for gastric ulcers. Pretreatment with DG at doses of 1, 2 and 4 ml/kg significantly (P<0.001) and dose dependently (25%, 53%, and 61% respectively) prevented indomethacin-induced gastric mucosal damage while ranitidine (50 mg/kg) showed 68% inhibition. [Table - 1]

Indomethacin also causes generation of reactive oxygen metabolites (such as superoxide anion, hydrogen peroxide, and hydroxyl radical), which damages the gastric tissue and causes ulcer formation. The pathogenesis of gastric mucosal lesions by indomethacin is associated with increased lipid peroxidation. Systemic administration of glutathione or SOD prevents gastric ulcers. In the present study, indomethacin-enhanced lipid peroxidation (233%), inhibited SOD (65%) and reduced glutathione (72%) concentration in the stomach tissue compared to normal rats. Treatment with DG caused dose-dependent reduction in the generation of MDA (37%, 48%, and 53%, respectively), while, enhanced the level of SOD (62%, 86% and 120%, respectively) and reduced glutathione (50%, 60%, and 82%, respectively) in gastric mucosal tissue. Reduced glutathione in the gastric mucosa acts as the major scavenger of the oxygen-derived free radicals. The standard drug, ranitidine corroborate these findings. Hence, it may be concluded that DG has preventive action on indomethacin-induced ulcer in rats. It is possible that the antioxidant effect of DG might also have played a role in the mechanism of antiulcer activity[6].

 
 » References Top

1.Jana U, Chattopadhyay RN, Shaw BP. Preliminary studies on antiinflammatory activity of Zingiber officinale Rosc, Vitex negundo Linn and Tinospora cordifolia (Willid) Miers in albino rats. Indian J Pharmacol 1999;31:232-3.  Back to cited text no. 1    
2.Sharma SS, Kochupillai V, Gupta SK, Seth SD, Gupta YK. Antiemetic efficacy of ginger ( Zingiber officinale ) against cisplatin-induced emesis in dogs. J Ethnopharmacol 1997;57:93-6.  Back to cited text no. 2    
3.Sharma PC, Yelne MB, Dennis TJ, editors. Database on Medicinal Plants used in Ayurveda. New Delhi: CCRS; 2001.  Back to cited text no. 3    
4.Sengupta K, ed. Bhabprakash. Kolkata: Purba Khanda; 2001.  Back to cited text no. 4    
5.Nadkarni KM. Indian Materia Medica. Vol. 1. Mumbai: Popular Prakashan; 1999.  Back to cited text no. 5    
6.Pandit S, Sur TK, Jana U, Bhattacharyya D, Debnath PK. Antiulcer effect of Shankha Bhasma in rats: A preliminary study. Indian J Pharmacol 2000;32:378-80.  Back to cited text no. 6    


Tables

[Table - 1]

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