| RESEARCH PAPER
|Year : 1999 | Volume
| Issue : 4 | Page : 266-274
Organotropic ultrastructural changes produced by monosodium glutamate in rats on Atherogenic diet: effect of S-allyl cysteine sulphoxide
KN Bopanna, R Balaraman, RS Nadig
Objectives: a) To study the effect of S-allyl cysteine sulphoxide (SACS) on histological and ultrastructural changes in liver, kidney, heart and brain produced by monosodium glutamate (MSG) in rats on atherogenic diet. b) To understand some cellular effects of MSG at higher dose in renal and hepatic tissues of rats on atherogenic diet.
Methods: Rats on atherogenic diet with MSG at two different doses received SACS for 30 days. At the end of 30 days, Na+ , K+ ATPase, 5' nucleotidase and membrane fluidisation assays were carried out in heart, kidney, liver and brain, respectively. Histological observation was also carried out for liver and kidney.
Results: SACS significantly decreased the Na+, K+ -ATPase and 5' nucleotidase activities in liver, kidney and heart in rats treated with MSG at both doses. There was no significant effect of SACS on brain Na+ ,K+ -ATPase and 5' nucleotidase in rats treated with both doses. Further, SACS significantly increased membrane fluidisation pattern in liver, kidney and heart in rats treated with MSG at both doses. MSG at higher dose produced sequence of damage in cellular organelles and membranes in renal and hepatic tissues. There was no significant effect of SACS on architectural, ultrastructural and histological changes induced by MSG in rats on atherogenic diet.
Conclusion: SACS significantly antagonised the reversible damage produced by MSG in rats onatherogenic diet by modulating Na+ , K+ -ATPase, 5' nucleotidase and membrane fluidisation process. Irreversible damage (oxidative stress) caused by MSG at higher dose on cell membrane and cell organelles of hepatic and renal tissues is not reverted by SACS in rats on atherogenic diet.
K N Bopanna
Source of Support: None, Conflict of Interest: None