| RESEARCH PAPER
|Year : 1999 | Volume
| Issue : 3 | Page : 187-189
Effect of calcium channel blockers on aluminium treated rat brain serine/threonine phosphoprotein phosphatase 2B
Basu Srabanti, Nag Chaudhuri Aditi
Objectives: To examine the effect of aluminium (Al) on different types of phosphatases in rat brain in the presence and absence of Ca 2+ channel blockers.
Methods: Phosphoprotein phosphatase activities were measured in brains of male Sprague Dawley rats treated with aluminium chloride (AlCl3 ) , Ca2+ channel blockers per se and AlCl3 with Ca2+ channel blockers for 40 days using standard techniques.
Results: Tyrosine phosphoprotein phosphatase (TPP) activity, activation of TPP by EDTA and activity of serine/threonine phosphoprotein phosphatase 2B (PP2B) were decreased by Al. Nifedipine treated rats regained the activities of TPP and PP2B and showed increased activity of serine/threonine phosphatase 2C (PP2C). Verapamil and diltiazem were ineffective in restoring the normal activities of the enzymes.
Conclusion: The restoration of the activity of PP2B by nifedipine indicates that there may be a competition between Al and Ca and between Al and Ca2+ channel blocker nifedipine. Hence the
Ca 2+ channel blocker nifedipine can be used to prevent Al 3+ toxicity.