IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 1180 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 »   Next article
 »   Previous article
 »   Table of Contents

Resource Links
 »   Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »   Citation Manager
 »   Access Statistics
 »   Reader Comments
 »   Email Alert *
 »   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1786    
    Printed83    
    Emailed0    
    PDF Downloaded139    
    Comments [Add]    

Recommend this journal

 

 RESEARCH PAPER
Year : 1998  |  Volume : 30  |  Issue : 3  |  Page : 195-198

Cultivation of mouse bone marrow-derived mast cells using Concanavalin-A stimulated splenocyte supernatant



Correspondence Address:
Singh Rashmi


Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and PermissionsRights and Permissions

Objective: To develop mast cells in vitro from mouse bone marrow using concanavalin-A stimulated splenocyte supernatant. Methods: Bone marrow cells (BMCs) were cultured in 1:1 (vol/vol) mixture of enriched medium (RPMI-1640 with 10% fetal calf serum with L-glutamine, 2 mM HEPES buffer, NaHCO3 (2.0 gm/L) and gentamicin (40 mg/L) and conditioned medium (conmed) derived from concanavalin-A (Con-A; 2.5 'g/ml) stimulated mouse splenocyte. Results: After culturing mouse bone marrow cells for seven days approximately 1 O-l 5% of cells began to show metachromatic granules with toluidine blue which suggested that these were mast cells. After 14 days of culture around 74-80% cells were identified as mast cells and heterogenous with respect to density of metachromatic granules. Maximum differentiation of bone marrow cells into mast cells were observed when conditioned medium and fresh medium dilution was 1:1 (vol/vol). when dilution of conditioned medium was 16x, 32x. 64x and 128x, lesser conversion of BMCs into mast cells were observed. Conclusion:The development of mast cells from bone marrow precursor cells in the presence of Con-A stimulated splenocyte supernatant suggested that T-cell derived growth factor had differentiated bone marrow cells into mast cells in vitro when its optimal concentration was used.






[PDF]*


        
Print this article     Email this article

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow