| RESEARCH PAPER
|Year : 1997 | Volume
| Issue : 4 | Page : 228-232
Role of benzodiazepine-GABA receptor complex in stress - induced modulation of leucocyte migration inhibition factor
PK Mediratta, KK Sharma
Objectives: To investigate the role of benzodiazepine-GABA receptor mechanisms in stress-induced
modulation of leucocyte migration inhibition factor (LMIF), a lymphokine released from sensitized lymphocytes.
Methods: Groups of male Wistar rats (n = 6 in each group) were subjected to restraint stress (RS) for 24 h at room temperature on day -1 (one day before), +1 or +13 (one or 13 days after) of sensitization with 0.5 ml of egg albumin (25 mg/ml) and 0.5 ml of complete Freund's adjuvant. Drugs/vehicle were injected i.p. just before submitting the animals to RS and LMIF activity was measured on day +14 by leucocyte migration inhibition (LMI) test.
Results: RS per se increased % LMI when given on day -1 but reduced it when administered on day +l and +13. Diazepam (5 mg/kg), muscimol (1 mg/kg) and baclofen (5 mg/kg) significantly attenuated the effect of RS on % LMI. The effect of diazepam was blocked by both flumazenil (10 mg/kg) and bicuculline (2 mg/kg); the latter also antagonised the effect of muscimol.
Conclusion: GABA-ergic mechanisms, mediated at both GABAA and GABAB receptors appear to play a role in the regulation of RS-induced changes in release activity of LMF.
P K Mediratta
Source of Support: None, Conflict of Interest: None