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 SHORT COMMUNICATION
Year : 1995  |  Volume : 27  |  Issue : 3  |  Page : 189-192

High performance liquid chromatographic method for simultaneous determination of ampicillin and sulbactam in biological samples



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S N Sulochana


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A reversed phase high performance liquid chromatographic (RP HPLC) method was developed for the simultaneous determination of ampicillin and sulbactam in serum and aqueous humor. A mixture of acetonitrile and tetrabutyl ammonium hydrogen sulfate was used as mobile phase while caffeine was the internal standard. The assay was rapid, simple and allowed a good linear range of detection from 0.5 (g to 20 (g for ampicillin and 0.5 (g to 10 (g for sulbactam at 230 nm. The analysis was done on serum and aqueous humor samples collected after giving intramuscular injection of 1 gm of ampicillin and 500 mg of sulbactam 60-140 min prior to surgery in patients undergoing cataract surgery. The assay was also performed in pooled human serum samples separately and together. The results were statistically analysed for the viability of the assay procedure.






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