| RESEARCH PAPER
|Year : 1986 | Volume
| Issue : 1 | Page : 14-18
Confirmation of the dopamine agonists activity of newly synthesised analogs using biochemical methods
Kuruvilla Alice, Furtell Richard, Uretsky Norman
1. Two newly synthesised sulfonium analogs have been tested for their dopamine agonist activity using biochemical methods.
2. Addition of dopamine and the analogs caused an increase in cyclic AMPproduction in a dose dependent manner in rat striatal slices.
3. Fluphenazine the dopamine antagonist when added to the incubation medium inhibited the increase in cyclic AMP levels produced by dopamine and the analogs.
4. Dopamine and apomorphine, the known agonists produced inhibition of 3H -ADTN binding to rat brain homogenates. Both the slufonium analogs also produced, inhibition of 3H - ADTN binding to rat brain homogenates.
5. The IC50 values of these two analogs (6.5 uM and 2uM) were higher than that of dopamine (188nM) and apomorphine (20nM).
Source of Support: None, Conflict of Interest: None