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 RESEARCH PAPER
Year : 1983  |  Volume : 15  |  Issue : 4  |  Page : 261-268

Immunological studies with the antigen of Setaria cervi (Nematoda ; Filariodea)


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K C Singhal


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The whole worm extract of Setaria cervi contains 52.7 mg Protein/g wet weight of the parasite.Fifty and 100% saturation of the extract with ammonium sulphate yielded two separate protein fractions. The antigen pattern in the parasite. was analyzed by various immunological tests such as gel diffusion, precipitin titration and passive cutaneous anaphylaxis (P. C. A.). Each rabbit was immunized by injecting a total of 3.7 mg protein according to the immunization schedule. Samples of sera collected on 32nd, 49th and 51st days were pooled in lot 1 and those collected on 55th, 57th and 59th days were pooled in lot II. Detection of antibodies by precipitin test showed that antibody titre reaches its peak in about 7 weeks and starts declining thereafter. The whole worm extract showed.4 precipitin bands with the immunized rabbit serum in gel diffusion. The two protein fractions obtained with 50 and 100% saturation with ammonium sulphate gave 3 and 2 bands respectively. Positive P. C. A. reaction was observed on guinea pigs with serum dilutions of 1 : 10 and 1 : 100. At a dilution of 1 : 1000 the reaction was negative. Positive reaction indicates the presence of reagin type of antibodies






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