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| RESEARCH LETTER |
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| Year : 2005 | Volume
: 37
| Issue : 6 | Page : 408-409 |
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Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity
P Jigna, N Rathish, C Sumitra
Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University,Rajkot-360 005., India
Correspondence Address:
C Sumitra
Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University,Rajkot-360 005.
India
  | 6 |
DOI: 10.4103/0253-7613.19085
How to cite this article:
Jigna P, Rathish N, Sumitra C. Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity. Indian J Pharmacol 2005;37:408-9 |
How to cite this URL:
Jigna P, Rathish N, Sumitra C. Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity. Indian J Pharmacol [serial online] 2005 [cited 2013 May 18];37:408-9. Available from: http://www.ijp-online.com/text.asp?2005/37/6/408/19085 |
In recent years, multiple drug resistance has developed due to indiscriminate use of existing antimicrobial drugs in the treatment of infectious diseases.[1] In addition to this, antibiotics are sometimes associated with adverse effects on the host-like hypersentivity. Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infectious diseases from other sources, such as plants.[2] Natural products of higher plants may be a new source of antimicrobial agents possibly with novel mechanisms of action.[3]
In vitro antimicrobial activity of aqueous and ethanol extracts of six medicinal plants used by traditional healers was examined. Microorganisms were obtained from National Chemical Laboratory (NCL), Pune, India. Microorganisms were maintained at 4°C on nutrient agar slants (for bacteria) and MGYP slants (for yeast).
Plant material (10 g) was extracted with 100 ml ethanol by subjecting it to agitation on rotary shaker (190-200 rpm) overnight, filtering it with muslin cloth and concentrating it to one-fifth of the volume. Crude aqueous extract was prepared by subjecting plant material (10 g) to slow heat for 6 hours and filtered through muslin cloth and concentrated it to one-fifth of the total volume. Antimicrobial activity of the ethanolic extracts was evaluated by agar well diffusion method[4] while aqueous extracts were evaluated by agar disc diffusion method.[5] The sensitivity of the extracts was measured using the diameter of the zone of inhibition.
Amongst the 12 extracts obtained from six plants, majority were active against Gram-positive bacteria [Table - 1], of which, the ethanol extracts were more active than aqueous extracts. The aqueous extract of Acyranthus aspera L. could inhibit Staphylococcus epidermidis to a certain extent; while rest of the bacteria was resistant to both aqueous and ethanolic extract of this plant. Aqueous extract of Calotropis gigantea L. could not inhibit any of the bacterial strains investigated except Proteus mirabilis. The ethanolic extract was most active against Klebsiella pneumoniae. Carissa congesta Wt. showed similar trend as that of Calotropis gigantea L. in which aqueous extract showed some activity only against Bacillus cereus, while ethanol extract could show antimicrobial activity to some extent. The ethanolic extract of Fagonia cretica L. and Rauvolfia serpentina L. could inhibit some of the bacterial strains studied to some degree while aqueous extract of both these plants did not show any activity at all. M. indica L. (both extracts) displayed remarkable activity. The most susceptible bacteria were Klebsiella pneumoniae  followed by Bacillus cereus, Escherichia More Details coli and Alcaligenes fecalis. Salmonella More Details typhimurium was the most resistant bacteria. All the aqueous extracts were inactive against C. tropicalis and only three ethanolic extracts viz. Calotropis gigantea L., Carissa congesta Wt. and Rauwolfia serpentina L. showed anticandidal activity . These observations are likely to be the result of the differences in cell wall structure between Gram-positive and Gram-negative bacteria, with Gram-negative outer membrane acting as a barrier to many environmental substances, including antibiotics.[6] The results can be compared with the standard antimicrobials [Table - 1].
In conclusion, the results of the present study support the folkloric usage of the studied plants and suggest that some of the plant extracts possess compounds with antimicrobial properties that can be further explored for antimicrobial activity.
| » References | |  |
| 1. | Service RF. Antibiotics that resist resistance. Science 1995;270:724-7.  [PUBMED] |
| 2. | Cordell GA. Biodiversity and drug discovery a symbiotic relationship . Phytochemistry 2000;55:463-80. [PUBMED] [FULLTEXT] |
| 3. | Barbour EK, Al Sharif M, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J Ethnopharmacol 2004;93:1-7. [PUBMED] [FULLTEXT] |
| 4. | Perez C, Paul M, Bazerque P. Antibiotic assay by agar-well diffusion method. Acta Biol Med Exp 1990;15:113-5. |
| 5. | Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6. [PUBMED] |
| 6. | Tortora GJ, Funke BR, Case CL. Microbiology: An Introduction. San Francisco: Benjamin Cummings; 2001. |
Tables
[Table - 1]
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